ST6GALNAC6 Monoklonaler Antikörper

ST6GALNAC6 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A7B7

Kat-Nr. : 66209-1-PBS

Synonyme

Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6, EC:2.4.99.-, GalNAc alpha-2,6-sialyltransferase VI, hST6GalNAc VI, RP11 203J24.3

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  WB analysis of COLO 320 using 66209-1-Ig (same clone as 66209-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 66209-1-Ig (same clone as 66209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 66209-1-Ig (same clone as 66209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66209-1-Ig (same clone as 66209-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ST6GALNAC6 antibody (66209-1-Ig, Clone: 1A7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66209-1-PBS bindet in WB, IHC, IF-P, ELISA ST6GALNAC6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ST6GALNAC6 fusion protein Ag8358
• Vollständiger Name: ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
• Berechnetes Molekulargewicht: 333aa,38 kDa; 374aa,41 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa
• GenBank-Zugangsnummer: BC007802
• Gene symbol: ST6GALNAC6
• Gene ID (NCBI): 30815
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.