ST8SIA1 Polyklonaler Antikörper

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• KD/KO Validated

ST8SIA1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 24918-1-AP

Synonyme

Alpha 2,8 sialyltransferase 8A, Ganglioside GD3 synthase, Ganglioside GT3 synthase, GD3S, Sialyltransferase 8A, Sialytransferase St8Sia I, SIAT8, SIAT8 A, SIAT8A, ST8SIA1, ST8SiaI

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Galerie der Validierungsdaten

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  WB analysis using 24918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24918-1-AP (ST8SIA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 24918-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MDA-MB-453s using 24918-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 24918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 24918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A375-Zellen, MCF-7-Zellen, MDA-MB-453s-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

24918-1-AP bindet in WB, IHC, IF, ELISA ST8SIA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ST8SIA1 fusion protein Ag20429
• Vollständiger Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
• Berechnetes Molekulargewicht: 356 aa, 41 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC126162
• Gene symbol: ST8SIA1
• Gene ID (NCBI): 6489
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST8SIA1 is a member of the ST family involved in the production of gangliosides. ST8SIA1 is strongly expressed in melanoma cell lines, adult and fetal brain, and to a lesser extent in adult and fetal lung. The abnormal expression of ST8SIA1 has been demonstrated to influence the metastasis of triple-negative breast cancer. (PMID: 34429775)

Publikationen

Species	Application	Title
	IF	Mol Oncol; Upregulation of cell surface GD3 ganglioside phenotype is associated with human melanoma brain metastasis.; Authors - Romela Irene Ramos
human	IHC	Front Pharmacol; Identifying an Immune-Related Gene ST8SIA1 as a Novel Target in Patients With Clear-Cell Renal Cell Carcinoma.; Authors - Xu Hu
human	IHC	Oncol Lett; ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway.; Authors - Shengjin Yu
mouse	WB	bioRxiv; Role of GD2 and its biosynthetic enzyme GD3 synthase in prostate cancer tumorigenesis; Authors - Aaqib M Bhat ; KO Validated
human	IHC	Front Physiol; Atlas of human dental pulp cells at multiple spatial and temporal levels based on single-cell sequencing analysis; Authors - Huihui Ren
human	WB	Front Cell Dev Biol; PRC2-dependent regulation of ganglioside expression during dedifferentiation contributes to the proliferation and migration of vascular smooth muscle cells; Authors - Norihiko Sasaki