ST8SIA1 Polyklonaler Antikörper

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• KD/KO Validated

ST8SIA1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 24918-1-AP

Synonyme

Alpha-2,8-sialyltransferase 8A, Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase, EC:2.4.3.8, Sialyltransferase St8Sia I, SIAT8

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  WB analysis using 24918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24918-1-AP (ST8SIA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24918-1-AP (ST8SIA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 24918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 24918-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 24918-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24918-1-AP (ST8SIA1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A375-Zellen, MCF-7-Zellen, MDA-MB-231-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

24918-1-AP bindet in WB, IHC, IF, ELISA ST8SIA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ST8SIA1 fusion protein Ag20429
• Vollständiger Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
• Berechnetes Molekulargewicht: 356 aa, 41 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC126162
• Gene symbol: ST8SIA1
• Gene ID (NCBI): 6489
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST8SIA1 is a member of the ST family involved in the production of gangliosides. ST8SIA1 is strongly expressed in melanoma cell lines, adult and fetal brain, and to a lesser extent in adult and fetal lung. The abnormal expression of ST8SIA1 has been demonstrated to influence the metastasis of triple-negative breast cancer. (PMID: 34429775)

Publikationen

Species	Application	Title
	IF	Mol Oncol; Upregulation of cell surface GD3 ganglioside phenotype is associated with human melanoma brain metastasis.; Authors - Romela Irene Ramos
human	IHC	Front Pharmacol; Identifying an Immune-Related Gene ST8SIA1 as a Novel Target in Patients With Clear-Cell Renal Cell Carcinoma.; Authors - Xu Hu
human	IHC	Oncol Lett; ST8SIA1 inhibits the proliferation, migration and invasion of bladder cancer cells by blocking the JAK/STAT signaling pathway.; Authors - Shengjin Yu
mouse	WB	bioRxiv; Role of GD2 and its biosynthetic enzyme GD3 synthase in prostate cancer tumorigenesis; Authors - Aaqib M Bhat ; KO Validated
mouse	WB	Sci Rep; GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior; Authors - Aaqib M Bhat
mouse,human	WB	Ann Med; Overexpression of ST8Sia1 inhibits tumor progression by TGF-β1 signaling in rectal adenocarcinoma and promotes the tumoricidal effects of CD8+ T cells by granzyme B and perforin; Authors - Chang Zhang