ST8SIA1 Polyklonaler Antikörper

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• KD/KO Validated

ST8SIA1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24918-1-PBS

Synonyme

Alpha-2,8-sialyltransferase 8A, Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase, EC:2.4.3.8, Sialyltransferase St8Sia I, SIAT8

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  WB analysis using 24918-1-AP (same clone as 24918-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24918-1-AP (ST8SIA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24918-1-PBS in a different storage buffer formulation.

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  WB analysis using 24918-1-AP (same clone as 24918-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24918-1-AP (ST8SIA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24918-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 24918-1-AP (same clone as 24918-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24918-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 24918-1-AP (same clone as 24918-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24918-1-AP (ST8SIA1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24918-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24918-1-AP (same clone as 24918-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24918-1-AP (ST8SIA1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24918-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24918-1-PBS bindet in WB, IHC, ELISA ST8SIA1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ST8SIA1 fusion protein Ag20429
• Vollständiger Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
• Berechnetes Molekulargewicht: 356 aa, 41 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC126162
• Gene symbol: ST8SIA1
• Gene ID (NCBI): 6489
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST8SIA1 is a member of the ST family involved in the production of gangliosides. ST8SIA1 is strongly expressed in melanoma cell lines, adult and fetal brain, and to a lesser extent in adult and fetal lung. The abnormal expression of ST8SIA1 has been demonstrated to influence the metastasis of triple-negative breast cancer. (PMID: 34429775)