ST8SIA2-Specific Polyklonaler Antikörper

ST8SIA2-Specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 19736-1-AP

Synonyme

Alpha 2,8 sialyltransferase 8B, HsT19690, Sialyltransferase 8B, Sialyltransferase X, Sialytransferase St8Sia II, SIAT8 B, SIAT8B, ST8SIA II, ST8SIA2, ST8SIA2-Specific, ST8SiaII, STX

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  WB analysis of mouse heart using 19736-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19736-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19736-1-AP (ST8SIA2-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 19736-1-AP (ST8SIA2-Specific Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 19736-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 19736-1-AP (ST8SIA2-Specific Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

19736-1-AP bindet in WB, IHC, IF, ELISA ST8SIA2-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: NM_006011
• Gene symbol: ST8SIA2
• Gene ID (NCBI): 8128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST8SIA2, also named as SIAT8B and STX, belongs to the glycosyltransferase 29 family. ST8SIA2 may transfer sialic acid through alpha-2,8-linkages to the alpha-2,3-linked and alpha-2,6-linked sialic acid of N-linked oligosaccharides of glycoproteins and may be involved in PSA (polysialic acid) expression. The antibody is specific to ST8SIA2.

Publikationen

Species	Application	Title
mouse	IF	Nat Neurosci; Single-cell genomics of the mouse olfactory cortex reveals contrasts with neocortex and ancestral signatures of cell type evolution; Authors - Sara Zeppilli
mouse,rat	WB,IHC	Oxid Med Cell Longev; Long Noncoding RNA TUG1 Aggravates Cerebral Ischemia/Reperfusion Injury by Acting as a ceRNA for miR-3072-3p to Target St8sia2.; Authors - Miao Chen
human	WB,IF	J Cell Sci; Prolyl endopeptidase is involved in the degradation of neural cell adhesion molecules in vitro.; Authors - Külli Jaako
mouse	WB	Brain Res; PSA modification of NCAM supports the survival of injured retinal ganglion cells in adulthood.; Authors - Natalia Lobanovskaya
human	IHC	Front Immunol; A novel ganglioside-related risk signature can reveal the distinct immune landscape of neuroblastoma and predict the immunotherapeutic response; Authors - Jiaxing Yang