STAM Polyklonaler Antikörper

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• KD/KO Validated

STAM Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 12434-1-AP

Synonyme

STAM, STAM 1, STAM1

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Galerie der Validierungsdaten

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  WB analysis using 12434-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Multi-cells using 12434-1-AP

  WB result of 12434-1-aP (STAM antibody).

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  WB analysis of mouse brain using 12434-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12434-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12434-1-AP (STAM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 12434-1-AP

  IP Result of anti-STAM (IP:12434-1-AP, 4ug; Detection:12434-1-AP 1:300) with PC-3 cells lysate 1440ug.

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  IHC staining of human testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 12434-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12434-1-AP (STAM antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12434-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12434-1-AP (STAM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12434-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using STAM antibody (12434-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, C2C12-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, MCF-7-Zellen, Multizellen, PC-3-Zellen, Raji-Zellen, SKOV-3-Zellen, Y79-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IF: See 8 publications below

Produktinformation

12434-1-AP bindet in WB, IP, IHC, IF, ELISA STAM und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STAM fusion protein Ag3112
• Vollständiger Name: signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC030586
• Gene symbol: STAM
• Gene ID (NCBI): 8027
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)

Publikationen

Species	Application	Title
human	WB	Nature; A phosphoinositide signalling pathway mediates rapid lysosomal repair; Authors - Jay Xiaojun Tan
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
human	WB	Mol Cell; Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.; Authors - Susanne Zellner
human	WB,IF	Cell Rep; TIM-1 Ubiquitination Mediates Dengue Virus Entry.; Authors - Ophélie Dejarnac ; KD Validated
		Development; Identification of STAM1 as a novel effector of ventral projection of spinal motor neurons.; Authors - Heejin Nam
human	IF,WB	PLoS Pathog; A combined EM and proteomic analysis places HIV-1 Vpu at the crossroads of retromer and ESCRT complexes: PTPN23 is a Vpu-cofactor.; Authors - Charlotte A Stoneham