STAMBPL1 Polyklonaler Antikörper

STAMBPL1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 27315-1-AP

Synonyme

ALMalpha, AMSH FP, AMSH like protease, AMSH LP, AMSHLP, KIAA1373, STAM binding protein like 1, STAMBPL1

---

• 

  WB analysis of human testis using 27315-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 27315-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27315-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 27315-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 27315-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, humanes Hodengewebe, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

27315-1-AP bindet in WB, IHC, IF, CoIP, ELISA STAMBPL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STAMBPL1 fusion protein Ag26308
• Vollständiger Name: STAM binding protein-like 1
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC010846
• Gene symbol: STAMBPL1
• Gene ID (NCBI): 57559
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Phytomedicine; A multi-omic analysis reveals that Gamabufotalin exerts anti-hepatocellular carcinoma effects by regulating amino acid metabolism through targeting STAMBPL1; Authors - Piao Zheng
human	WB,IHC	Mol Carcinog; STAMBPL1, transcriptionally regulated by SREBP1, promotes malignant behaviors of hepatocellular carcinoma cells via Wnt/β-catenin signaling pathway; Authors - Junyi Jin
human	WB	Transl Oncol; STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression; Authors - Xiang Yang
human,mouse	WB,IF,CoIP	Cell Rep; A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma; Authors - Hongli Zhang ; KO Validated