STAR Polyklonaler Antikörper

STAR Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(68)

Kat-Nr. : 12225-1-AP

Synonyme

STARD1, START domain-containing protein 1

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  WB analysis using 12225-1-AP

  Pig adrenal gland lysates were subjected to SDS PAGE followed by western blot with 12225-1-AP (STAR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 12225-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12225-1-AP (STAR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12225-1-AP (STAR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12225-1-AP (STAR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse testis using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 12225-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 12225-1-AP (STAR Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse testis using 12225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using STAR antibody (12225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 12225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 12225-1-AP (STAR antibody) at dilution of 1:400 and CoraLite®647-conjugated AffiniPure F(ab, CoraLite®555 CL555-17178 (TNP1 antibody, orange) and CoraLite® Plus 488 CL488-13720 (BOULE antibody, green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Nebennierengewebe, humanes Nebennierengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Leberkarzinomgewebe, humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe
• Erfolgreiche Detektion in IF-Fro: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 54 publications below
• IHC: See 10 publications below
• IF: See 14 publications below

Produktinformation

12225-1-AP bindet in WB, IHC, IF-P, IF-Fro, ELISA STAR und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STAR fusion protein Ag2862
• Vollständiger Name: steroidogenic acute regulatory protein
• Berechnetes Molekulargewicht: 285 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC010550
• Gene symbol: STAR
• Gene ID (NCBI): 6770
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.

Publikationen

Species	Application	Title
human	WB	Transl Res; Induction of autophagy by Beclin-1 in granulosa cells contributes to follicular progesterone elevation in ovarian endometriosis.; Authors - Ying Ding
human	IHC	Clin Transl Med; Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma.; Authors - Qian Hao
mouse	WB	Int J Nanomedicine; La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.; Authors - Lu Yuan
mouse	WB	Ecotoxicol Environ Saf; FDX1 regulates leydig cell ferroptosis mediates PM2.5-induced testicular dysfunction of mice; Authors - Lipeng Li
mouse	WB	Environ Pollut; Prenatal exposure to propylparaben at human-relevant doses accelerates ovarian aging in adult mice.; Authors - Milu Li
pig	WB	Biol Res; Unraveling the impact of hyperleptinemia on female reproduction: insights from transgenic pig model; Authors - Muhammad Ameen Jamal