STAR Monoklonaler Antikörper

STAR Monoklonal Antikörper für WB, IF-P, IF-Fro, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF-P, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G6E6

Kat-Nr. : 67130-1-PBS

Synonyme

1G6E6, STARD1, START domain-containing protein 1

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  WB analysis of pig adrenal gland using 67130-1-Ig (same clone as 67130-1-PBS)

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  WB analysis using 67130-1-Ig (same clone as 67130-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human adrenal gland using 67130-1-Ig (same clone as 67130-1-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat adrenal gland using 67130-1-Ig (same clone as 67130-1-PBS)

  rat adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 67130-1-Ig (same clone as 67130-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67130-1-Ig (same clone as 67130-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 67130-1-Ig (same clone as 67130-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 67130-1-Ig (same clone as 67130-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 67130-1-Ig (STAR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (67130-1-Ig, Clone: 1G6E6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67130-1-Ig (same clone as 67130-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 67130-1-Ig (STAR antibody) at dilution of 1:400, CoraLite®555 CL555-17178 (TNP1 antibody, orange) and CoraLite® Plus 488 CL488-13720 (BOULE antibody, green). This data was developed using the same antibody clone with 67130-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67130-1-PBS bindet in WB, IF-P, IF-Fro, Indirect ELISA STAR und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STAR fusion protein Ag28698
• Vollständiger Name: steroidogenic acute regulatory protein
• Berechnetes Molekulargewicht: 285 aa, 32 kDa
• Beobachtetes Molekulargewicht: 28-32 kDa
• GenBank-Zugangsnummer: BC010550
• Gene symbol: STAR
• Gene ID (NCBI): 6770
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue