STARD7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

STARD7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Rezensionen (1)

Kat-Nr. : 15689-1-AP

Synonyme

GTT1, StAR-related lipid transfer protein 7, mitochondrial, START domain-containing protein 7

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  WB analysis using 15689-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15689-1-AP (STARD7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15689-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15689-1-AP (STARD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 15689-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15689-1-AP (STARD7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 15689-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15689-1-AP (STARD7 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, K-562-Zellen, L02-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below

Produktinformation

15689-1-AP bindet in WB, IHC, ELISA STARD7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STARD7 fusion protein Ag8224
• Vollständiger Name: StAR-related lipid transfer (START) domain containing 7
• Berechnetes Molekulargewicht: 295 aa, 35 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC008894
• Gene symbol: STARD7
• Gene ID (NCBI): 56910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STARD7, also known as gestational trophoblastic tumor gene-1 (GTT1), is a lipid transfer protein that specifically binds and transports phosphatidylcholine (PC) between cellular membranes. It is a member of the START (Steroidogenic Acute Regulatory protein-related lipid transfer) domain-containing protein family, which is involved in intracellular lipid transport and metabolism. (PMID: 23507753)

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	Nat Commun; Intronic ATTTC repeat expansions in STARD7 in familial adult myoclonic epilepsy linked to chromosome 2.; Authors - Mark A Corbett
human	WB	EMBO J; PARL partitions the lipid transfer protein STARD7 between the cytosol and mitochondria.; Authors - Shotaro Saita
mouse	WB	EMBO Mol Med; Loss of the mitochondrial i-AAA protease YME1L leads to ocular dysfunction and spinal axonopathy.; Authors - Hans-Georg Sprenger
human	WB	J Lipid Res; A search for ceramide binding proteins using bifunctional lipid analogs yields CERT-related protein StarD7.; Authors - Svenja Bockelmann ; KO Validated
human	WB	iScience; Lipopolysaccharide induces placental mitochondrial dysfunction in murine and human systems by reducing MNRR1 levels via a TLR4-independent pathway; Authors - Neeraja Purandare

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Elsa (Verified Customer) (05-06-2019)

The antibody works well, a band at the expected molecular weight is shown. No unspecificity.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/2500
• Cell Tissue Type: mouse microglia cell line