STARD7 Rekombinanter Antikörper

STARD7 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242760A3

Kat-Nr. : 85131-4-RR

Synonyme

242760A3, GTT1, StAR-related lipid transfer protein 7, mitochondrial, START domain-containing protein 7

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  WB analysis using 85131-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85131-4-RR (STARD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 85131-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85131-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85131-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85131-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85131-4-RR against Human STARD7 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, HT-1080.Zellen, K-562-Zellen, MCF-7-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85131-4-RR bindet in WB, IHC, ELISA STARD7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: STARD7 fusion protein Ag8224
• Vollständiger Name: StAR-related lipid transfer (START) domain containing 7
• Berechnetes Molekulargewicht: 295 aa, 35 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC008894
• Gene symbol: STARD7
• Gene ID (NCBI): 56910
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STARD7, also known as gestational trophoblastic tumor gene-1 (GTT1), is a lipid transfer protein that specifically binds and transports phosphatidylcholine (PC) between cellular membranes. It is a member of the START (Steroidogenic Acute Regulatory protein-related lipid transfer) domain-containing protein family, which is involved in intracellular lipid transport and metabolism. (PMID: 23507753)