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Phospho-STAT1 (Ser727) Rekombinanter Antikörper

Phospho-STAT1 (Ser727) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240897D10

Kat-Nr. : 80683-7-PBS

Synonyme

STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-STAT1 (Ser727) Recombinant antibody (80683-7-RR)

    WB analysis using 80683-7-RR (same clone as 80683-7-PBS)

    Non treated and IFN gamma treated HeLa cells were subjected to SDS PAGE followed by western blot with 80683-7-RR (Phospho-STAT1 (Ser727) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80683-7-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

80683-7-PBS bindet in WB, Indirect ELISA Phospho-STAT1 (Ser727) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name signal transducer and activator of transcription 1, 91kDa
Berechnetes Molekulargewicht 83 kDa
Beobachtetes Molekulargewicht84-91 kDa
GenBank-ZugangsnummerBC002704
Gene symbol STAT1
Gene ID (NCBI) 6772
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 activating phosphorylation at Ser727 is p38-dependant duringlipotoxic stress. MLK3 activation triggers a MAPK signaling cascade resulting in STAT1 Ser727 phosphorylation. The phosphorylation of STAT1 Ser727 increases its nuclear localization, and transcriptional activity. (PMID: 28262979).