STAT1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

STAT1 Rekombinant Antikörper für WB, IHC, IF/ICC, IP, ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2F16

Kat-Nr. : 82016-1-RR

Synonyme

2F16, ISGF 3, Signal transducer and activator of transcription 1-alpha/beta, STAT91, Transcription factor ISGF-3 components p91/p84

---

• 

  WB analysis using 82016-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82016-1-RR (STAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 82016-1-RR

  WB result of STAT1 antibody (82016-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells.

• 

  IP experiment of PC-3 using 82016-1-RR

  IP result of anti-STAT1 (IP:82016-1-RR, 4ug; Detection:82016-1-RR 1:10000) with PC-3 cells lysate 2000 ug.

• 

  IHC staining of human ovary tumor using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 82016-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 82016-1-RR

  Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (82016-1-RR, Clone: 2F16 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  ChIP experiment of HT-1080 using 82016-1-RR

  Chromatin was prepared from HT-1080 cells either non-treated or treated with hIFN-γ (50 ng/ml, 30min). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of STAT1 (82016-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

• 

  Affinity and Kinetic Characterization of 82016-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82016-1-RR against Human STAT1 were performed. The affinity constant is 0.501 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, Maus-Kolongewebe, Mausmilzgewebe, Ratten-Kolongewebe, Rattenmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: IFN alpha treated HeLa cells
• Erfolgreiche Detektion in ChIP-qPCR: IFN-γ -HT-1080 (50 ng/ml, 30min) HT-1080 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

82016-1-RR bindet in WB, IHC, IF/ICC, IP, ELISA, ChIP-qPCR STAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: STAT1 fusion protein Ag0199
• Vollständiger Name: signal transducer and activator of transcription 1, 91kDa
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 84 kDa
• GenBank-Zugangsnummer: BC002704
• Gene symbol: STAT1
• Gene ID (NCBI): 6772
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Various ligands, including interferons- and - , EGF, PDGF and IL-6, can activate STAT1. STAT1 protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.

Publikationen

Species	Application	Title
human	WB	Noncoding RNA Res; LncRNA RNF144A-AS1 gene polymorphisms and their influence on lung cancer patients in the Chinese Han population; Authors - Hongjiao Wu
human	WB	Cell Biochem Biophys; FOXA1 Transcriptional Repression of PLSCR1 Inhibits Tongue Squamous Cell Carcinoma Progression; Authors - Jinxin Wang