STAT2 Rekombinanter Antikörper

STAT2 Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242685A1

Kat-Nr. : 80061-5-PBS

Synonyme

242685A1, ISGF 3, ISGF3, P113, STAT 2

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  WB analysis using 80061-5-RR (same clone as 80061-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80061-5-RR (STAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80061-5-PBS in a different storage buffer formulation.

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  FC experiment of Ramos using 80061-5-RR (same clone as 80061-5-PBS)

  1x10^6 Ramos cells were intracellularly stained with 0.25 ug STAT2 Recombinant antibody (80061-5-RR, Clone:242685A1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80061-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80061-5-RR

  Biolayer interferometry (BLl) kinetic assays of 80061-5-RR against Human STAT2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

80061-5-PBS bindet in WB, FC (Intra), Indirect ELISA STAT2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: STAT2 fusion protein Ag10168
• Vollständiger Name: signal transducer and activator of transcription 2, 113kDa
• Berechnetes Molekulargewicht: 851 aa, 98 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC051284
• Gene symbol: STAT2
• Gene ID (NCBI): 6773
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IF stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.