STAT2 Monoklonaler Antikörper
STAT2 Monoklonal Antikörper für FC (Intra), IF
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus, Ratte
Anwendung
IF, FC (Intra)
Konjugation
CoraLite®594 Fluorescent Dye
CloneNo.
1G12C4
Kat-Nr. : CL594-66485
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in IF | Ramos-Zellen |
Erfolgreiche Detektion in FC | Ramos-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Immunfluoreszenz (IF) | IF : 1:200-1:800 |
Durchflusszytometrie (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
CL594-66485 bindet in IF, FC (Intra) STAT2 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | STAT2 fusion protein Ag10168 |
Vollständiger Name | signal transducer and activator of transcription 2, 113kDa |
Berechnetes Molekulargewicht | 851 aa, 98 kDa |
Beobachtetes Molekulargewicht | 113 kDa |
GenBank-Zugangsnummer | BC051284 |
Gene symbol | STAT2 |
Gene ID (NCBI) | 6773 |
Konjugation | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3. |
Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IF stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.
Protokolle
Produktspezifische Protokolle | |
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IF protocol for CL594 STAT2 antibody CL594-66485 | Protokoll herunterladen |
FC protocol for CL594 STAT2 antibody CL594-66485 | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |