STAT3 Monoklonaler Antikörper

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• KD/KO Validated

STAT3 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G2D12

Kat-Nr. : 60199-1-PBS

Synonyme

3G2D12, Acute-phase response factor, APRF, HIES, STAT 3

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  WB analysis of HeLa using 60199-1-Ig (same clone as 60199-1-PBS)

  WB result of STAT3 antibody (60199-1-Ig, 1:3000) with si-Control and si-STAT3 transfected HeLa cells. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60199-1-Ig (same clone as 60199-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60199-1-Ig (same clone as 60199-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60199-1-Ig (same clone as 60199-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60199-1-Ig (same clone as 60199-1-PBS)

  IP result of anti-STAT3 (IP:60199-1-Ig, 5ug; Detection:60199-1-Ig 1:2000) with HeLa cells lysate 1400ug. This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60199-1-Ig(STAT3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60199-1-Ig (same clone as 60199-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT3 antibody (60199-1-Ig, Clone: 3G2D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 60199-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60199-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA STAT3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STAT3 fusion protein Ag0360
• Vollständiger Name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Berechnetes Molekulargewicht: 770 aa, 88 kDa
• Beobachtetes Molekulargewicht: 85-88 kDa
• GenBank-Zugangsnummer: BC000627
• Gene symbol: STAT3
• Gene ID (NCBI): 6774
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.