STAT3 Rekombinanter Antikörper

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• KD/KO Validated

STAT3 Rekombinant Antikörper für WB, IHC, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

3H1

Kat-Nr. : 80149-1-PBS

Synonyme

3H1, Acute-phase response factor, APRF, HIES, STAT 3

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  WB analysis using 80149-1-RR (same clone as 80149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80149-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80149-1-RR (same clone as 80149-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80149-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 80149-1-RR (same clone as 80149-1-PBS)

  WB result of STAT3 antibody (80149-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT3 transfected HeLa cells. This data was developed using the same antibody clone with 80149-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80149-1-RR (same clone as 80149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80149-1-RR (STAT3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80149-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80149-1-RR (same clone as 80149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80149-1-RR (STAT3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80149-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HepG2 using 80149-1-RR (same clone as 80149-1-PBS)

  Chromatin was prepared from HepG2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of STAT3 (80149-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 80149-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80149-1-PBS bindet in WB, IHC, Indirect ELISA, ChIP-qPCR STAT3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Berechnetes Molekulargewicht: 770 aa, 88 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC000627
• Gene symbol: STAT3
• Gene ID (NCBI): 6774
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3.