STAT3 Rekombinanter Antikörper

STAT3 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB

Konjugation

Unkonjugiert

CloneNo.

3H1

Kat-Nr. : 80149-1-RR

Synonyme

Acute phase response factor, APRF, FLJ20882, HIES, STAT3

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Galerie der Validierungsdaten

• 

  WB analysis using 80149-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 80149-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80149-1-RR (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 80149-1-RR

  WB result of STAT3 antibody (80149-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT3 transfected HeLa cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80149-1-RR bindet in WB STAT3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Berechnetes Molekulargewicht: 770 aa, 88 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC000627
• Gene symbol: STAT3
• Gene ID (NCBI): 6774
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3.