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STAT5A/B Polyklonaler Antikörper

STAT5A/B Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

Kat-Nr. : 13179-1-AP

Synonyme

STAT5A, MGF, STAT5



Geprüfte Anwendungen

Erfolgreiche Detektion in WBK-562-Zellen, 3T3-L1-Zellen, Daudi-Zellen, DU 145-Zellen, SGC-7901-Zellen
Erfolgreiche IPK-562-Zellen, 3T3-L1-Zellen
Erfolgreiche Detektion in IHChumanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in FC (Intra)MCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13179-1-AP bindet in WB, IHC, IF, FC (Intra), IP, ELISA STAT5A/B und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte, Rind, Kuh
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen STAT5A/B fusion protein Ag3846
Vollständiger Name signal transducer and activator of transcription 5A
Berechnetes Molekulargewicht 794 aa, 92 kDa
Beobachtetes Molekulargewicht 90-95 kDa
GenBank-ZugangsnummerBC027036
Gene symbol STAT5A
Gene ID (NCBI) 6776
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells. This antibody is a rabbit polyclonal antibody raised against 445-794 aa of human STAT5A, the homology between this segment and the corresponding segment of human STAT5B is 92%. It shows that this antibody can cross-react with STAT5B.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for STAT5A/B antibody 13179-1-APProtokoll herunterladen
IHC protocol for STAT5A/B antibody 13179-1-APProtokoll herunterladenl
IP protocol for STAT5A/B antibody 13179-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Carbohydr Polym

Structural characterizations and antiaging activities of hydrolyzed low-molecular-weight polysaccharides from Polygoni Multiflori Radix Praeparata

Authors - Wei Xu
humanWB

Front Immunol

Exhausted signature and regulatory network of NK cells in myasthenia gravis

Authors - Qing Zhang
humanWB

Front Immunol

Elevated levels of exogenous prolactin promote inflammation at the maternal-fetal interface via the JAK2/STAT5B signaling axis

Authors - Alycia Williams
ratWB,IF

Chemosphere

MEHP promotes liver fibrosis by down-regulating STAT5A in BRL-3A hepatocytes.

Authors - Yuezhu Zhang
  • KD Validated
humanWB

Endocrinology

A Novel Mechanism of hPRL-G129R, A Prolactin Antagonist, Inhibits Human Breast Cancer Cell Proliferation and Migration

Authors - Ruonan Li
humanWB,IHC

Front Oncol

In-depth analysis of the expression and functions of signal transducers and activators of transcription in human ovarian cancer

Authors - Xiaodi Gong