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STAT5A Monoklonaler Antikörper

STAT5A Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F10F6

Kat-Nr. : 66459-1-Ig

Synonyme

MGF, STAT5, STAT5A

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of various lysates using STAT5A Monoclonal antibody (66459-1-Ig)

    WB analysis using 66459-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66459-1-Ig (STAT5A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using STAT5A Monoclonal antibody (66459-1-Ig)

    WB analysis of U-937 using 66459-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 66459-1-Ig (STAT5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STAT5A Monoclonal antibody (66459-1-Ig)

    IF Staining of HepG2 using 66459-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT5A antibody (66459-1-Ig, Clone: 1F10F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STAT5A Monoclonal antibody (66459-1-Ig)

    IF Staining of HepG2 using 66459-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STAT5A antibody (66459-1-Ig, Clone: 1F10F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBRAW 264.7-Zellen, Hausschwein-Milzgewebe, Rattenmilzgewebe, U-937-Zellen
Erfolgreiche Detektion in IFHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:16000
Immunfluoreszenz (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

66459-1-Ig bindet in WB, IF, ELISA STAT5A und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen STAT5A fusion protein Ag19546
Vollständiger Name signal transducer and activator of transcription 5A
Berechnetes Molekulargewicht 794 aa, 92 kDa
Beobachtetes Molekulargewicht 95 kDa
GenBank-ZugangsnummerBC027036
Gene symbol STAT5A
Gene ID (NCBI) 6776
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.

Protokolle

Produktspezifische Protokolle
WB protocol for STAT5A antibody 66459-1-IgProtokoll herunterladen
IF protocol for STAT5A antibody 66459-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

J Clin Invest

Intrinsic RIG-I restrains STAT5 activation to modulate anti-tumor activity of CD8+ T cells

Authors - Xinyi Jiang
View Article