STAT5A Monoklonaler Antikörper

STAT5A Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA, N/A

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA, N/A

Konjugation

Unkonjugiert

CloneNo.

1F10F6

Kat-Nr. : 66459-1-PBS

Synonyme

1F10F6, MGF, STAT5

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  WB analysis using 66459-1-Ig (same clone as 66459-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66459-1-Ig (STAT5A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66459-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 66459-1-Ig (same clone as 66459-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 66459-1-Ig (STAT5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66459-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP50852-1

  Cytometric bead array in cell lysate using MP50852-1, Phospho-STAT5A (Ser726) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66459-1-PBS. Detection antibody: 60596-1-PBS. Cell lysate: Non-treated TF-1 and Calyculin A treated TF-1 (30μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

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  IF Staining of HepG2 using 66459-1-Ig (same clone as 66459-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT5A antibody (66459-1-Ig, Clone: 1F10F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66459-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66459-1-Ig (same clone as 66459-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STAT5A antibody (66459-1-Ig, Clone: 1F10F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66459-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 66459-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 66459-1-Ig against Human STAT5A was performed. The affinity constant is 7.00 nM.

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Geprüfte Anwendungen

Produktinformation

66459-1-PBS bindet in WB, IF/ICC, Indirect ELISA, N/A STAT5A und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STAT5A fusion protein Ag19546
• Vollständiger Name: signal transducer and activator of transcription 5A
• Berechnetes Molekulargewicht: 794 aa, 92 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC027036
• Gene symbol: STAT5A
• Gene ID (NCBI): 6776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.