STAT5A/B Polyklonaler Antikörper

STAT5A/B Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12071-1-AP

Synonyme

STAT5, STAT5A/B, STAT5B

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Galerie der Validierungsdaten

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  WB analysis using 12071-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12071-1-AP (STAT5A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 12071-1-AP

  IP result of anti-STAT5A/B(IP:12071-1-AP, 4ug; Detection:12071-1-AP 1:4000) with HeLa cells lysate 1720 ug.

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  IHC staining of human cervical cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12071-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12071-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12071-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12071-1-AP (STAT5A/B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12071-1-AP (STAT5A/B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12071-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12071-1-AP (STAT5A/B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12071-1-AP

  Immunofluorescent analysis of HepG2 cells using 12071-1-AP (STAT5A/B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MOLT-4-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

12071-1-AP bindet in WB, IP, IHC, IF, ELISA STAT5A/B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STAT5A/B fusion protein Ag2709
• Vollständiger Name: signal transducer and activator of transcription 5B
• Berechnetes Molekulargewicht: 787 aa, 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC020868
• Gene symbol: STAT5B
• Gene ID (NCBI): 6777
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Although this antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT5B, the homology between this segment and the corresponding segment of human STAT5A is 92%. It shows that this antibody can cross-react with STAT5A.

Publikationen

Species	Application	Title
rat	WB	Biomed Pharmacother; Kuijieling regulates the differentiation of Treg and Th17 cells to ameliorate experimental colitis in rats.; Authors - Yu Long
rat	WB, IP, IF	Toxicol Appl Pharmacol; PMID: 26493931; Authors - Furong Zhang
rat	WB	Pharm Biol; Yiguanjian decoction inhibits macrophage M1 polarization and attenuates hepatic fibrosis induced by CCl4/2-AAF.; Authors - Ying Xu
mouse	WB	Biochem Biophys Res Commun; 1, 25(OH)2D3-induced interaction of vitamin D receptor with p50 subunit of NF-κB suppresses the interaction between KLF5 and p50, contributing to inhibition of LPS-induced macrophage proliferation.; Authors - Dong Ma
mouse	WB	Biomed Pharmacother; Efficacy of WWQ-131, a highly selective JAK2 inhibitor, in mouse models of myeloproliferative neoplasms; Authors - Huan Ge
rat		J Ethnopharmacol; Freeze-dried powder of daylily bud improves bromocriptine-induced lactation disorder in rats via JAK2/STAT5 pathway; Authors - Shang Guo