STAT6 Monoklonaler Antikörper

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STAT6 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E5B8

Kat-Nr. : 66717-1-PBS

Synonyme

2E5B8, IL-4 Stat, Signal transducer and activator of transcription 6, STAT 6, STAT6B

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  WB analysis using 66717-1-Ig (same clone as 66717-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66717-1-Ig (STAT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66717-1-Ig (same clone as 66717-1-PBS)

  WB result of STAT6 antibody (66717-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected HeLa cells. This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66717-1-Ig (same clone as 66717-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66717-1-Ig (STAT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66717-1-Ig (same clone as 66717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66717-1-Ig (same clone as 66717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66717-1-Ig (same clone as 66717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human appendicitis using 66717-1-Ig (same clone as 66717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66717-1-Ig (STAT6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66717-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66717-1-PBS bindet in WB, IHC, ELISA STAT6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STAT6 fusion protein Ag0410
• Vollständiger Name: signal transducer and activator of transcription 6, interleukin-4 induced
• Berechnetes Molekulargewicht: 100 kDa
• Beobachtetes Molekulargewicht: 95 kDa
• GenBank-Zugangsnummer: BC005823
• Gene symbol: STAT6
• Gene ID (NCBI): 6778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.