STAT6 Rekombinanter Antikörper

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• KD/KO Validated

STAT6 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4F19

Kat-Nr. : 82630-1-PBS

Synonyme

4F19, IL-4 Stat, Signal transducer and activator of transcription 6, STAT 6, STAT6B

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  WB analysis using 82630-1-RR (same clone as 82630-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82630-1-RR (STAT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82630-1-RR (same clone as 82630-1-PBS)

  WB result of STAT6 antibody (82630-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected HeLa cells. This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 82630-1-RR (same clone as 82630-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82630-1-RR (STAT6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 82630-1-RR (same clone as 82630-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT6 antibody (82630-1-RR, Clone: 4F19 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82630-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82630-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA STAT6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: signal transducer and activator of transcription 6, interleukin-4 induced
• Berechnetes Molekulargewicht: 94 kDa
• Beobachtetes Molekulargewicht: 105-110 kDa
• GenBank-Zugangsnummer: BC075852
• Gene symbol: STAT6
• Gene ID (NCBI): 6778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.