STAU1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

STAU1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Kat-Nr. : 14225-1-AP

Synonyme

Double-stranded RNA-binding protein Staufen homolog 1, STAU

---

• 

  WB analysis of human brain using 14225-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14225-1-AP

  WB result of STAU1 antibody (14225-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAU1 transfected HepG2 cells.

• 

  WB analysis of mouse brain using 14225-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of K-562 using 14225-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14225-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14225-1-AP (STAU1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14225-1-AP

  IP result of anti-STAU1 (IP:14225-1-AP, 4ug; Detection:14225-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of mouse pancreas using 14225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14225-1-AP (STAU1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 14225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14225-1-AP (STAU1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14225-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14225-1-AP (STAU1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 14225-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using STAU1 antibody (14225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HepG2-Zellen, K-562-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 4 publications below

Produktinformation

14225-1-AP bindet in WB, IHC, IF/ICC, IP, RIP, ELISA STAU1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STAU1 fusion protein Ag5464
• Vollständiger Name: staufen, RNA binding protein, homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC050432
• Gene symbol: STAU1
• Gene ID (NCBI): 6780
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. There are two isoforms of STAU1 protein and the molecular weight of two isoforms are repectively 63 kDa and 55 kDa

Publikationen

Species	Application	Title
human	WB	Nature; hiCLIP reveals the in vivo atlas of mRNA secondary structures recognized by Staufen 1.; Authors - Yoichiro Sugimoto ; KD Validated
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
	RIP	Mol Cell; Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation.; Authors - Shih-Han Lee
mouse,human	WB,RIP,IHC	Nat Commun; Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk; Authors - Pingting Ying
human	WB	Neuron; Axonal Transport of TDP-43 mRNA Granules Is Impaired by ALS-Causing Mutations.; Authors - Nael H Alami
human	WB	Nucleic Acids Res; Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.; Authors - Evan P Booy