STAU1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

STAU1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242179D12

Kat-Nr. : 84822-4-RR

Synonyme

242179D12, Double-stranded RNA-binding protein Staufen homolog 1, STAU

---

• 

  WB analysis of HepG2 using 84822-4-RR

  WB result of STAU1 antibody (84822-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAU1 transfected HepG2 cells.

• 

  WB analysis using 84822-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84822-4-RR (STAU1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84822-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84822-4-RR against Human STAU1 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HepG2-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84822-4-RR bindet in WB, ELISA STAU1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: STAU1 fusion protein Ag5464
• Vollständiger Name: staufen, RNA binding protein, homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC050432
• Gene symbol: STAU1
• Gene ID (NCBI): 6780
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.