STBD1 Polyklonaler Antikörper

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• KD/KO Validated

STBD1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 11842-1-AP

Synonyme

Genethonin 1, GENEX3414, GENX 3414, starch binding domain 1, STBD1

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 11842-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11842-1-AP (STBD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11842-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11842-1-AP (STBD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11842-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11842-1-AP (STBD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11842-1-AP

  IP Result of anti-STBD1 (IP:11842-1-AP, 4ug; Detection:11842-1-AP 1:1000) with A549 cells lysate 1600ug.

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  IHC staining of human colon cancer using 11842-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11842-1-AP (STBD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11842-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11842-1-AP (STBD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, A549-Zellen, HeLa-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Produktinformation

11842-1-AP bindet in WB, IP, IHC, IF, ELISA STBD1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STBD1 fusion protein Ag2481
• Vollständiger Name: starch binding domain 1
• Berechnetes Molekulargewicht: 41 kDa
• Beobachtetes Molekulargewicht: 35-43 kDa
• GenBank-Zugangsnummer: BC022301
• Gene symbol: STBD1
• Gene ID (NCBI): 8987
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.

Publikationen

Species	Application	Title
mouse	IF	Cell; Glycogen accumulation and phase separation drives liver tumor initiation.; Authors - Qingxu Liu
mouse	WB	Acta Pharmacol Sin; Asiatic acid alleviates ischemic myocardial injury in mice by modulating mitophagy- and glycophagy-based energy metabolism.; Authors - Fan Qiu
mouse	WB,IF	J Cell Sci; Stbd1 promotes glycogen clustering during endoplasmic reticulum stress and supports survival of mouse myoblasts; Authors - Andria A. Lytridou ; KD Validated
mouse	WB	Mol Endocrinol; Transgenic muscle specific Nor-1 expression regulates multiple pathways that effect adiposity, metabolism and endurance.; Authors - Pearen Michael A MA
	WB,IF	J Cell Sci; Mouse Stbd1 is N-myristoylated and affects ER-mitochondria association and mitochondrial morphology.; Authors - Anthi Demetriadou
mouse,rat	IF,IP	J Biol Chem; Starch binding domain-containing protein 1/genethonin 1 is a novel participant in glycogen metabolism.; Authors - Jiang Sixin S