Stanniocalcin 2 Polyklonaler Antikörper

Stanniocalcin 2 Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10314-1-AP

Synonyme

stanniocalcin 2, Stanniocalcin related protein, STC 2, STC related protein, STC2, STCRP

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Galerie der Validierungsdaten

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  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 10314-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using 10314-1-AP (Stanniocalcin 2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of U2OS using 10314-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Stanniocalcin 2 antibody (10314-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Produktinformation

10314-1-AP bindet in WB, IHC, IF, ELISA Stanniocalcin 2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Stanniocalcin 2 fusion protein Ag0359
• Vollständiger Name: stanniocalcin 2
• Berechnetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC000658
• Gene symbol: STC2
• Gene ID (NCBI): 8614
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stanniocalcin (STC) is a family of secreted glycoprotein hormones that originally discovered in the corpuscles of Stannius, an endocrine gland of fish. STC1 and STC2, two homologues of STC family, are reported to involve in calcium and phosphate homeostasis. It is expressed in a wide variety of tissues such as kidney, spleen, heart, and pancreas. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC2 overexpression could promote tumor cell proliferation, invasion and metastasis in prostate cancer, ovarian cancer or neuroblastoma. STC2 is also vital for cytoprotective properties when exposed to ER stress and hypoxia.

Publikationen

Species	Application	Title
human	WB,IHC	Eur Urol; Identification of stanniocalcin 2 as prognostic marker in renal cell carcinoma.; Authors - Meyer Hellmuth-A HA
human	WB	Proc Natl Acad Sci U S A; Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.; Authors - Lei Shi
human	IHC	Genomics Proteomics Bioinformatics; miRNA-mRNA Regulatory Network and Factors Associated with Prediction of Prognosis in Hepatocellular Carcinoma.; Authors - Bo Hu
human	IHC	Int J Cancer; Additive effect of the AZGP1, PIP, S100A8, and UBE2C molecular biomarkers improves outcome prediction in breast carcinoma.; Authors - Parris Toshima Z TZ
human	IHC	Endocr Relat Cancer; Tumor suppressor functions of DAXX through histone H3.3/H3K9me3 pathway in pancreatic NETs.; Authors - Hiroki Ueda
human	WB	Sci Rep; Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma.; Authors - Dachang Liu