STEAP1 Polyklonaler Antikörper

STEAP1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 20199-1-AP

Synonyme

Metalloreductase STEAP1, PRSS24, STEAP, STEAP1

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  WB analysis of A431 using 20199-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20199-1-AP (STEAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 20199-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20199-1-AP (STEAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 20199-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 20199-1-AP (STEAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 20199-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20199-1-AP (STEAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 20199-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20199-1-AP (STEAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, DU 145-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

20199-1-AP bindet in WB, IHC, ELISA STEAP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STEAP1 fusion protein Ag14134
• Vollständiger Name: six transmembrane epithelial antigen of the prostate 1
• Berechnetes Molekulargewicht: 339 aa, 40 kDa
• Beobachtetes Molekulargewicht: 38-40 kDa
• GenBank-Zugangsnummer: BC011802
• Gene symbol: STEAP1
• Gene ID (NCBI): 26872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Cell Death Dis; METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition.; Authors - Yue Zhu
human	WB	Cancer Biol Ther; STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2; Authors - Dong Zhang