STEAP3 Polyklonaler Antikörper

STEAP3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 28478-1-AP

Synonyme

dudlin 2, Dudulin 2, hpHyde, hTSAP6, Metalloreductase STEAP3, pHyde, STEAP family member 3, STEAP3, STMP3, TSAP6

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  WB analysis using 28478-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28478-1-AP (STEAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 28478-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 28478-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 28478-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 28478-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 28478-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28478-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STEAP3 antibody (28478-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, Mauslebergewebe, PC-12-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe, humanes Urothelkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

28478-1-AP bindet in WB, IHC, IF/ICC, ELISA STEAP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STEAP3 fusion protein Ag29528
• Vollständiger Name: STEAP family member 3
• Berechnetes Molekulargewicht: 488 aa, 55 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC042150
• Gene symbol: STEAP3
• Gene ID (NCBI): 55240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STEAP3 (Six-Transmembrane Epithelial Antigen of Prostate 3) is also named as TSAP6, Dudulin-2 and pHyde, and belongs to the STEAP family. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).

Publikationen

Species	Application	Title
mouse	WB	FASEB J; Prolonged tamoxifen treatment induces iron deposition and ferroptosis in the liver; Authors - Jianxin Yang
human	IHC	BMC Pulm Med; PCR array analysis reveals a novel expression profile of ferroptosis-related genes in idiopathic pulmonary fibrosis; Authors - Chenyou Shen
rat	WB,IF	Int Immunopharmacol; STEAP3-SLC39A8-mediated microglia ferroptosis involved in neurotoxicity in rats after exposure to lead and cadmium combined; Authors - Yuxin Sun
bovine	WB	J Dairy Sci; Heat shock protein B1-mediated ferroptosis regulates mitochondrial dysfunction in adipose tissue of ketotic dairy cows; Authors - Yunhui Fan
human	WB,IHC	Biol Direct; TFAP2C-mediated transcriptional activation of STEAP3 promotes lung squamous cell carcinoma progression by regulating the β-catenin pathway; Authors - Tong Sun