STIM2 Rekombinanter Antikörper

STIM2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250016E8

Kat-Nr. : 85727-1-RR

Synonyme

KIAA1482, Stromal interaction molecule 2

---

• 

  WB analysis using 85727-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85727-1-RR (STIM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85727-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85727-1-RR against Human STIM2 were performed. The affinity constant is 0.774 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, BxPC-3-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85727-1-RR bindet in WB, ELISA STIM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: STIM2 fusion protein Ag15530
• Vollständiger Name: stromal interaction molecule 2
• Berechnetes Molekulargewicht: 754 aa, 85 kDa
• Beobachtetes Molekulargewicht: 85-115 kDa
• GenBank-Zugangsnummer: BC146661
• Gene symbol: STIM2
• Gene ID (NCBI): 57620
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STIM2, also named as KIAA1482, functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. STIM2 may inhibit STIM1-mediated Ca2+ influx. STIM2 has 2 isoforms 105kd and 115kd with Differential levels of phosphorylation.