STIP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

STIP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 15218-1-AP

Synonyme

Hop, Hsc70/Hsp90 organizing protein, Hsc70/Hsp90-organizing protein, IEF SSP 3521, P60

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  WB analysis using 15218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15218-1-AP (STIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15218-1-AP (STIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15218-1-AP

  WB result of STIP1 antibody (15218-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STIP1 transfected HEK-293 cells.

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  IP experiment of Jurkat using 15218-1-AP

  IP result of anti-STIP1 (IP:15218-1-AP, 4ug; Detection:15218-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human liver cancer using 15218-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15218-1-AP (STIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 15218-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15218-1-AP (STIP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15218-1-AP

  Immunofluorescent analysis of MCF-7 cells, using STIP1 antibody 15218-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, human normal colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15218-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA STIP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STIP1 fusion protein Ag7388
• Vollständiger Name: stress-induced-phosphoprotein 1
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC002987
• Gene symbol: STIP1
• Gene ID (NCBI): 10963
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).

Publikationen

Species	Application	Title
human	WB	Nature; Structure of Hsp90-Hsp70-Hop-GR reveals the Hsp90 client-loading mechanism.; Authors - Ray Yu-Ruei Wang
human	WB	Cancer Manag Res; STIP1 Regulates Proliferation and Migration of Lung Adenocarcinoma Through JAK2/STAT3 Signaling Pathway.; Authors - Xiangjun Guo ; KD Validated
human	WB	Hum Cell; Stress-induced phosphoprotein 1 facilitates breast cancer cell progression and indicates poor prognosis for breast cancer patients.; Authors - Lizhi Lin
human	WB	Mol Biol Cell; Mechanism of chaperone recruitment and retention on mitochondrial precursors; Authors - Szymon Juszkiewicz
human	WB	Gene; Systematic study of stress-inducible protein 1 (Stip1) in male reproductive system and its expression during stress response.; Authors - Yanwei Wang
mouse,human	WB,IHC,IF	Exp Cell Res; Tumor-promoting action of ubiquitin protease 43 in gastric cancer progression through deubiquitination and stabilization of stress-inducible phosphoprotein 1; Authors - Zijun Guo