STK10 Polyklonaler Antikörper

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• KD/KO Validated

STK10 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 25471-1-AP

Synonyme

EC:2.7.11.1, LOK, Lymphocyte oriented kinase, Lymphocyte-oriented kinase, PRO2729

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  WB analysis of SKOV-3 using 25471-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 25471-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 25471-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 25471-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 25471-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 25471-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, A549-Zellen, COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

25471-1-AP bindet in WB, IHC, ELISA STK10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STK10 fusion protein Ag22169
• Vollständiger Name: serine/threonine kinase 10
• Berechnetes Molekulargewicht: 968 aa, 112 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC070077
• Gene symbol: STK10
• Gene ID (NCBI): 6793
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lymphocyte-oriented kinase deficiency encoded by the serine/threonine kinase 10 (STK10) gene correlates with the intracellular adhesion molecule 1 (ICAM-1)/lymphocyte function associated antigen 1 (LFA-1) complex in aspirin hypersensitivity(PMID:21905501). STK 10 is also named as LOK and expressed as a 130-kDa protein, which was detected predominantly in lymphoid organs such as spleen, thymus, and bone marrow, in contrast to other mammalian members of the STE20 family(PMID:9278426).

Publikationen

Species	Application	Title
human	WB	Front Immunol; The enhancement of immunoactivity induced by immunogenic cell death through serine/threonine kinase 10 inhibition: a potential therapeutic strategy; Authors - Xiaoli Xia ; KD Validated
		Clin Cancer Res; Combined CDK4/6 and ERK1/2 inhibition enhances anti-tumor activity in NF1-associated plexiform neurofibroma; Authors - Alyssa C Flint

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jared (Verified Customer) (08-21-2019)

This antibody is an all-star antibody. I can even re use it a couple of times, and it still works. potent stuff. Picture would not upload... There is one strong band, and it shows virtually nothing in the lane where I CRISPR'd out LOK aka STK10 in the LOK band area. There is a non-specific band, but that is located clearly out of the range of where the LOK band is located. Non-specific band at approx 200kd. LOK for me runs at around 140kd.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: 293T and H1299

STK10 Antibody Western Blot, validation (1:3000 dilution) in 293T and H1299 (Cat no:25471-1-AP)