STK10 Polyklonaler Antikörper

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• KD/KO Validated

STK10 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25471-1-PBS

Synonyme

EC:2.7.11.1, LOK, Lymphocyte oriented kinase, Lymphocyte-oriented kinase, PRO2729

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  WB analysis of SKOV-3 using 25471-1-AP (same clone as 25471-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 25471-1-AP (same clone as 25471-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 25471-1-AP (same clone as 25471-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 25471-1-AP (same clone as 25471-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 25471-1-AP (same clone as 25471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 25471-1-AP (same clone as 25471-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25471-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25471-1-PBS bindet in WB, IHC, Indirect ELISA STK10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STK10 fusion protein Ag22169
• Vollständiger Name: serine/threonine kinase 10
• Berechnetes Molekulargewicht: 968 aa, 112 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC070077
• Gene symbol: STK10
• Gene ID (NCBI): 6793
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lymphocyte-oriented kinase deficiency encoded by the serine/threonine kinase 10 (STK10) gene correlates with the intracellular adhesion molecule 1 (ICAM-1)/lymphocyte function associated antigen 1 (LFA-1) complex in aspirin hypersensitivity(PMID:21905501). STK 10 is also named as LOK and expressed as a 130-kDa protein, which was detected predominantly in lymphoid organs such as spleen, thymus, and bone marrow, in contrast to other mammalian members of the STE20 family(PMID:9278426).