STK11/LKB1 Monoklonaler Antikörper

STK11/LKB1 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H8E4

Kat-Nr. : 66719-1-Ig

Synonyme

LKB1, PJS, serine/threonine kinase 11, STK11, STK11/ LKB1

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Galerie der Validierungsdaten

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  WB analysis using 66719-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66719-1-Ig (STK11/LKB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66719-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66719-1-Ig (STK11/LKB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 66719-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66719-1-Ig (STK11/LKB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 66719-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66719-1-Ig (STK11/LKB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66719-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66719-1-Ig (STK11/LKB1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66719-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66719-1-Ig (STK11/LKB1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66719-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66719-1-Ig (STK11/LKB1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 66719-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STK11/LKB1 antibody (66719-1-Ig, Clone: 1H8E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66719-1-Ig bindet in WB, IHC, IF, ELISA STK11/LKB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STK11/LKB1 fusion protein Ag13436
• Vollständiger Name: serine/threonine kinase 11
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 45-49 kDa
• GenBank-Zugangsnummer: BC007981
• Gene symbol: STK11
• Gene ID (NCBI): 6794
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.