STK25 Polyklonaler Antikörper

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• KD/KO Validated

STK25 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 25821-1-AP

Synonyme

Ste20 like kinase, SOK-1, SOK1, SOK 1, Serine/threonine-protein kinase 25

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  WB analysis using 25821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25821-1-AP (STK25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25821-1-AP (STK25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 25821-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25821-1-AP (STK25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25821-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25821-1-AP (STK25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 25821-1-AP

  IP result of anti-STK25 (IP:25821-1-AP, 4ug; Detection:25821-1-AP 1:4000) with mouse brain tissue lysate 1840 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, HepG2-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IP: See 1 publications below

Produktinformation

25821-1-AP bindet in WB, IP, ELISA STK25 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STK25 fusion protein Ag22964
• Vollständiger Name: serine/threonine kinase 25 (STE20 homolog, yeast)
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC015793
• Gene symbol: STK25
• Gene ID (NCBI): 10494
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Signal Transduct Target Ther; The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease; Authors - Chandana Thimme Gowda
mouse	IP	Cell Mol Gastroenterol Hepatol; Targeted Delivery of Stk25 Antisense Oligonucleotides to Hepatocytes Protects Mice Against Nonalcoholic Fatty Liver Disease.; Authors - Emmelie Cansby
human,mouse	WB	Cell Mol Gastroenterol Hepatol; Antagonizing STK25 Signaling Suppresses the Development of Hepatocellular Carcinoma Through Targeting Metabolic, Inflammatory, and Pro-Oncogenic Pathways.; Authors - Yeshwant Kurhe ; KD Validated
human	WB	J Exp Clin Cancer Res; STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer.; Authors - Fan Wu ; KD Validated
human	WB	Mol Carcinog; Downregulation of STK25 promotes autophagy via the Janus kinase 2/signal transducer and activator of transcription 3 pathway in colorectal cancer.; Authors - Jiangbo Chen
rat	WB	Cell Mol Neurobiol; Lanthanum Chloride Induces Axon Abnormality Through LKB1-MARK2 and LKB1-STK25-GM130 Signaling Pathways.; Authors - Zeli Song