STK39 Rekombinanter Antikörper

STK39 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230382G12

Kat-Nr. : 83081-3-PBS

Synonyme

230382G12, DCHT, PASK, SPAK, Ste 20 related kinase

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  IHC staining of mouse brain using 83081-3-RR (same clone as 83081-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83081-3-RR (STK39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83081-3-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83081-3-RR (same clone as 83081-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STK39 antibody (83081-3-RR, Clone: 230382G12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83081-3-PBS in a different storage buffer formulation.

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  FC experiment of Daudi using 83081-3-RR (same clone as 83081-3-PBS)

  1x10^6 Daudi cells were intracellularly stained with 0.25 ug Anti-Human STK39 (83081-3-RR, Clone:230382G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83081-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83081-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83081-3-RR against Human STK39 were performed. The affinity constant is 1.21 nM.

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Geprüfte Anwendungen

Produktinformation

83081-3-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA STK39 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: STK39 fusion protein Ag34503
• Vollständiger Name: serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
• Berechnetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC166614
• Gene symbol: STK39
• Gene ID (NCBI): 27347
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.