MST1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MST1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Kat-Nr. : 22245-1-AP

Synonyme

KRS2, MST 1, MST1, serine/threonine kinase 4, STE20 like kinase MST1, STK4, STK4/MST1, YSK3

---

Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 22245-1-AP

  WB result of MST1 antibody (22245-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MST1 transfected HepG2 cells.

• 

  WB analysis using 22245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22245-1-AP (MST1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 22245-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22245-1-AP (MST1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 22245-1-AP

  IP Result of anti-MST1 (IP:22245-1-AP, 4ug; Detection:22245-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human prostate cancer using 22245-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22245-1-AP (MST1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22245-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22245-1-AP (MST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 22245-1-AP

  Immunofluorescent analysis of HepG2 cells using 22245-1-AP (MST1 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C2C12-Zellen, HepG2-Zellen, Jurkat-Zellen, Mausmilzgewebe, Ramos-Zellen, Rattenlebergewebe, Rattenmilzgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

22245-1-AP bindet in WB, IP, IHC, IF, ELISA MST1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MST1 fusion protein Ag17738
• Vollständiger Name: serine/threonine kinase 4
• Berechnetes Molekulargewicht: 487 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC093768
• Gene symbol: STK4
• Gene ID (NCBI): 6789
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.

Publikationen

Species	Application	Title
mouse	IF,WB	Cell; Glycogen accumulation and phase separation drives liver tumor initiation.; Authors - Qingxu Liu
human	WB	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen
mouse	IHC	Cell Death Differ; Hippo kinases MST1 and MST2 control the differentiation of the epididymal initial segment via the MEK-ERK pathway.; Authors - Chenling Meng
mouse	WB,IHC	J Am Soc Nephrol; Tubule-Specific Mst1/2 Deficiency Induces CKD via YAP and Non-YAP Mechanisms.; Authors - Chunhua Xu ; KO Validated
human	WB,IHC,IF	Oncogene; Deacetylation of tumor-suppressor MST1 in Hippo pathway induces its degradation through HBXIP-elevated HDAC6 in promotion of breast cancer growth.; Authors - L Li
human	WB	Cell Death Differ; Suppressor of hepatocellular carcinoma RASSF1A activates autophagy initiation and maturation.; Authors - Wenjiao Li