STMN2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

STMN2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Rezensionen (1)

Kat-Nr. : 10586-1-AP

Synonyme

Protein SCG10, SCG10, SCGN10, SGC10, Stathmin 2, stathmin like 2, STMN2

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Galerie der Validierungsdaten

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  WB analysis using 10586-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10586-1-AP (STMN2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10586-1-AP

  IP result of anti-STMN2(IP:10586-1-AP, 4ug; Detection:10586-1-AP 1:2000) with mouse brain tissue lysate 2080 ug.

• 

  IHC staining of mouse brain using 10586-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10586-1-AP (STMN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10586-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10586-1-AP (STMN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 10586-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (10586-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

10586-1-AP bindet in WB, IP, IHC, IF, ELISA STMN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STMN2 fusion protein Ag0929
• Vollständiger Name: stathmin-like 2
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 18-21 kDa
• GenBank-Zugangsnummer: BC006302
• Gene symbol: STMN2
• Gene ID (NCBI): 11075
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)

Publikationen

Species	Application	Title
mouse	WB	Neuron; Molecular substrates of altered axonal growth and brain connectivity in a mouse model of schizophrenia.; Authors - Jun Mukai
mouse	WB	Proc Natl Acad Sci U S A; SCG10 is a JNK target in the axonal degeneration pathway.; Authors - Shin Jung Eun JE ; KD Validated
mouse	WB	J Cell Biol; SARM1 acts downstream of neuroinflammatory and necroptotic signaling to induce axon degeneration.; Authors - Kwang Woo Ko
mouse	WB	J Cell Biol; A neuroprotective agent that inactivates prodegenerative TrkA and preserves mitochondria.; Authors - Konstantin Feinberg
zebrafish	IHC	Elife; Retrograde Ret signaling controls sensory pioneer axon outgrowth.; Authors - Adam Tuttle
mouse	IHC,IF	J Neurosci; CNGA3: a target of spinal nitric oxide/cGMP signaling and modulator of inflammatory pain hypersensitivity.; Authors - Heine Sandra S

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tatyana (Verified Customer) (01-09-2023)

Good signal both in WB and ICC on neuroblastoma cells (neuronal cell line). Was used 1:1000 in both applications. Gave some non-specific bands in WB. ICC - 5% goat serum / PBST WB - 4% milk / TBST

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / SH-SY5Y cells

STMN2 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:10586-1-AP)

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