STMN2 Rekombinanter Antikörper

STMN2 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

230110D5

Kat-Nr. : 82925-1-RR

Synonyme

230110D5, SCG 10, SCG10, SCGN 10, SCGN10

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  WB analysis using 82925-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82925-1-RR (STMN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 82925-1-RR

  IP result of anti-STMN2 (IP:82925-1-RR, 4ug; Detection:82925-1-RR 1:5000) with mouse brain tissue lysate 1800 ug.

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  IHC staining of mouse brain using 82925-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-1-RR (STMN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82925-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82925-1-RR (STMN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 82925-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STMN2 antibody (82925-1-RR, Clone: 230110D5 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 82925-1-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (82925-1-RR, Clone: 230110D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 82925-1-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug STMN2 Recombinant antibody (82925-1-RR, Clone:230110D5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82925-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82925-1-RR against Human STMN2 were performed. The affinity constant is 1.29 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, fetales humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82925-1-RR bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA STMN2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: STMN2 fusion protein Ag0929
• Vollständiger Name: stathmin-like 2
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 18-21 kDa
• GenBank-Zugangsnummer: BC006302
• Gene symbol: STMN2
• Gene ID (NCBI): 11075
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)