STOML2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

STOML2 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

CloneNo.

1A2E9

Kat-Nr. : 60052-1-Ig

Synonyme

1A2E9, EPB72 like protein 2, EPB72-like protein 2, Paraprotein target 7, Paratarg-7

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  WB analysis of pig brain using 60052-1-Ig

  Pig, rat, and mouse brain tissues were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60052-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 60052-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 60052-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 60052-1-Ig

  IP result of anti-STOML2 (IP:60052-1-Ig, 4ug; Detection:60052-1-Ig 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human stomach cancer using 60052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 60052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 60052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 60052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 60052-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using STOML2 antibody (60052-1-Ig, Clone: 1A2E9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, HepG2-Zellen, MCF-7-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Endometriumkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 3 publications below

Produktinformation

60052-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA STOML2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STOML2 fusion protein Ag0363
• Vollständiger Name: stomatin (EPB72)-like 2
• Berechnetes Molekulargewicht: 356 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC002442
• Gene symbol: STOML2
• Gene ID (NCBI): 30968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.

Publikationen

Species	Application	Title
human	WB	Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima ; KD Validated
human	WB,IP	Mol Cell; β-hydroxybutyrate facilitates mitochondrial-derived vesicle biogenesis and improves mitochondrial functions; Authors - Min Tang
human	WB	Mol Cell Proteomics; An informatics-assisted label-free approach for personalized tissue membrane proteomics: case study on colorectal cancer.; Authors - Han Chia-Li CL
mouse,human	IP,IF	Int J Mol Sci; The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.; Authors - Meiyu Cheng
human	IHC	Int J Cancer; Novel prognostic protein markers of resectable pancreatic cancer identified by coupled shotgun and targeted proteomics using formalin-fixed paraffin-embedded tissues.; Authors - Takadate Tatsuyuki T
human	WB	Cell Death Dis; Stomatin-like protein 2 regulates survivin expression in non-small cell lung cancer cells through β-catenin signaling pathway.; Authors - Cheng-Ta Yang ; KD Validated