STOML2 Monoklonaler Antikörper

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• KD/KO Validated

STOML2 Monoklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A2E9

Kat-Nr. : 60052-1-PBS

Synonyme

1A2E9, EPB72 like protein 2, EPB72-like protein 2, Paraprotein target 7, Paratarg-7

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  WB analysis of pig brain using 60052-1-Ig (same clone as 60052-1-PBS)

  Pig, rat, and mouse brain tissues were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60052-1-Ig (same clone as 60052-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60052-1-Ig (same clone as 60052-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 60052-1-Ig (same clone as 60052-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 60052-1-Ig (same clone as 60052-1-PBS)

  IP result of anti-STOML2 (IP:60052-1-Ig, 4ug; Detection:60052-1-Ig 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60052-1-Ig (same clone as 60052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 60052-1-Ig (same clone as 60052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 60052-1-Ig (same clone as 60052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 60052-1-Ig (same clone as 60052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 60052-1-Ig (same clone as 60052-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using STOML2 antibody (60052-1-Ig, Clone: 1A2E9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60052-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA STOML2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STOML2 fusion protein Ag0363
• Vollständiger Name: stomatin (EPB72)-like 2
• Berechnetes Molekulargewicht: 356 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC002442
• Gene symbol: STOML2
• Gene ID (NCBI): 30968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.