STRA8 Monoklonaler Antikörper

STRA8 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

1D7E3

Kat-Nr. : 68071-1-Ig

Synonyme

STRA8

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Galerie der Validierungsdaten

• 

  WB analysis of human testis using 68071-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 68071-1-Ig (STRA8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit testis using 68071-1-Ig

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 68071-1-Ig (STRA8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 68071-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 68071-1-Ig (STRA8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hodengewebe, Kaninchenhodengewebe, Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

68071-1-Ig bindet in WB, IF, ELISA STRA8 und zeigt Reaktivität mit human, Kaninchen, Maus

• Getestete Reaktivität: human, Kaninchen, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STRA8 fusion protein Ag29680
• Vollständiger Name: stimulated by retinoic acid gene 8 homolog (mouse)
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 43-60 kda
• GenBank-Zugangsnummer: NM_182489
• Gene symbol: STRA8
• Gene ID (NCBI): 346673
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stimulated by retinoic acid 8 (Stra8), one of genes induced by retinoic acid (RA), is required for the meiotic initiation of male spermatogenesis. Stimulated by Retinoic Acid Gene 8 (STRA8) protein has been proposed as the molecular effector of Retinoic Acid (RA) involved in meiosis-inductive activity. Stra8 is a 45-kDa vertebrate-specific gene, without significant homologous proteins, expressed in the cytoplasm and nuclei of germ cells between mitosis and meiosis and presents exclusively in the embryonic and postnatal gonads of mammals. (PMID: 30106128, PMID: 31253359)

Publikationen

Species	Application	Title
mouse	WB,IF	Environ Pollut; Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling; Authors - Yifan Hong
mouse	WB	J Hazard Mater; Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice; Authors - Tianxin Zhao