STRN Rekombinanter Antikörper

STRN Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242532D8

Kat-Nr. : 85058-4-PBS

Synonyme

242532D8, SG2NA, Striatin

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  WB analysis using 85058-4-RR (same clone as 85058-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85058-4-RR (STRN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85058-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85058-4-RR (same clone as 85058-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85058-4-RR (STRN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85058-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85058-4-RR (same clone as 85058-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85058-4-RR (STRN antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85058-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 85058-4-RR (same clone as 85058-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STRN antibody (85058-4-RR, Clone: 242532D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85058-4-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 85058-4-RR (same clone as 85058-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STRN antibody (85058-4-RR, Clone: 242532D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85058-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85058-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85058-4-RR against Human STRN were performed. The affinity constant is 0.433 nM.

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Geprüfte Anwendungen

Produktinformation

85058-4-PBS bindet in WB, IHC, IF-P, Indirect ELISA STRN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: STRN fusion protein Ag15984
• Vollständiger Name: striatin, calmodulin binding protein
• Berechnetes Molekulargewicht: 780 aa, 86 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC106879
• Gene symbol: STRN
• Gene ID (NCBI): 6801
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STRN (Striatin), a 780-amino acid protein, was identified and cloned more than a decade ago. In adult mammals, striatin is mainly expressed in neurons of both the central and peripheral nervous systems with very high levels of expression in the striatum, hence its name. STRN-ALK fusion may be a potential therapeutic target in the aggressive forms of thyroid cancer (PMID: 24475247).