STRN3 Polyklonaler Antikörper

STRN3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 23966-1-AP

Synonyme

Striatin-3, Striatin3, Striatin 3, SG2NA, PPP2R6B

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  WB analysis using 23966-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23966-1-AP (STRN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 23966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 23966-1-AP (STRN3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 23966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 23966-1-AP (STRN3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maus-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

23966-1-AP bindet in WB, IHC, IF, ELISA STRN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STRN3 fusion protein Ag21091
• Vollständiger Name: striatin, calmodulin binding protein 3
• Berechnetes Molekulargewicht: 797 aa, 87 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC126221
• Gene symbol: STRN3
• Gene ID (NCBI): 29966
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Mol Biol Cell; Spatial proximity of proteins surrounding zyxin under force-bearing conditions.; Authors - Joleen S Cheah
human	WB,IF	STAR Protoc; Protocol using lentivirus to establish THP-1 suspension cell lines for immunostaining and confocal microscopy; Authors - Wangsheng Ji
human	WB	Genes Genomics; LncRNA KTN1-AS1 facilitates esophageal squamous cell carcinoma progression via miR-885-5p/STRN3 axis; Authors - Liying Chen
human	WB	Adv Sci (Weinh); TRAF3IP3 Induces ER Stress-Mediated Apoptosis with Protective Autophagy to Inhibit Lung Adenocarcinoma Proliferation; Authors - Guang Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joleen (Verified Customer) (05-04-2019)

Works well with Western Blot but requires long exposure >100s. Some nonspecific band but still not dirty.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

STRN3 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:23966-1-AP)