STS Polyklonaler Antikörper

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• KD/KO Validated

STS Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17870-1-AP

Synonyme

ARSC, ARSC1, Arylsulfatase C, ASC, ES, SSDD, Steroid sulfatase, Steryl sulfatase, Steryl sulfate sulfohydrolase, STS

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 17870-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17870-1-AP (STS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 17870-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17870-1-AP (STS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human paracancerous tissue of hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human paracancerous tissue of hysteromyoma using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous tissue of hysteromyoma using 17870-1-AP (STS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17870-1-AP (STS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17870-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17870-1-AP (STS antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 17870-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17870-1-AP (STS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Lungengewebe, humanes Nierengewebe, humanes Uterusmyomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

17870-1-AP bindet in WB, IHC, IF, ELISA STS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STS fusion protein Ag12353
• Vollständiger Name: steroid sulfatase (microsomal), isozyme S
• Berechnetes Molekulargewicht: 583 aa, 65 kDa
• Beobachtetes Molekulargewicht: 63-65 kDa
• GenBank-Zugangsnummer: BC075030
• Gene symbol: STS
• Gene ID (NCBI): 412
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STS(Steroid sulfatase) is also named as ASC(arylsulfatase C), ARSC1 and belongs to the sulfatase family. It is expressed in human liver and is responsible for the hydrolysis of many estrogen and hydroxysteroid sulfates, including dehydroepiandrosterone (DHEA)-sulfate and β-estradiol(E2)-3-sulfate(PMID:19589875). This enzyme has also been documented to occur in many other tissues, including skin, lung, ovary, and adrenal gland. The estimated molecular mass of expressed human STS is 63-85 kDa which may represent different levels of glycosylation in the different tissues, as STS is known to be a glycoprotein and the full length protein has a signal peptide with 21 amino acids(PMID:17604157).

Publikationen

Species	Application	Title
human	WB	Clin Cancer Res; Steroid Sulfatase Stimulates Intracrine Androgen Synthesis and is a Therapeutic Target for Advanced Prostate Cancer.; Authors - Cameron M Armstrong ; KD Validated
mouse, rat	WB	Sci Rep; Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application.; Authors - Jiansha Li
human	WB	Mol Cell Biochem; Resveratrol and its methoxy derivatives modulate the expression of estrogen metabolism enzymes in breast epithelial cells by AhR down-regulation.; Authors - Barbara Licznerska
mouse	WB,IF	J Biomed Res; Suppressed estrogen supply via extra-ovarian progesterone receptor membrane component 1 in menopause.; Authors - Sang R Lee
mouse	IF	Life Sci Alliance; Macrophage Sult2b1 promotes pathological neovascularization in age-related macular degeneration; Authors - Yafang Wang