STT3A Monoklonaler Antikörper

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STT3A Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E2B12

Kat-Nr. : 66581-1-PBS

Synonyme

ITM1, Integral membrane protein 1, EC:2.4.99.18, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A, B5

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  WB analysis of THP-1 using 66581-1-Ig (same clone as 66581-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66581-1-Ig (STT3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66581-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66581-1-Ig (same clone as 66581-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66581-1-Ig (STT3A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66581-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66581-1-Ig (same clone as 66581-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66581-1-Ig (STT3A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66581-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66581-1-PBS bindet in WB, IHC, Indirect ELISA STT3A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STT3A fusion protein Ag27072
• Vollständiger Name: STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 705 aa, 81 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC020965
• Gene symbol: STT3A
• Gene ID (NCBI): 3703
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STT3A, also named as Integral membrane protein 1, belongs to the STT3 family. STT3A is expressed at high levels in placenta, liver, muscle and pancreas, and at very low levels in brain, lung and kidney. STT3A is a catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient post-translational glycosylation and mediate glycosylation of sites that have been skipped by STT3A. There are two isoforms of STT3A with molecular weight of 81 and 70 kDa. 66581-1-Ig antibody detects a protein around 65-70 kDa in SDS-PAGE which is similar to papers published. (PMID: 32005703, 25135935)