STT3B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

STT3B Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 15323-1-AP

Synonyme

SIMP, STT3 B, STT3B

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Galerie der Validierungsdaten

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  WB analysis using 15323-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15323-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15323-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15323-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15323-1-AP (STT3B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 15323-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15323-1-AP (STT3B Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HepG2 using 15323-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15323-1-AP (STT3B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, humanes Hirngewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15323-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA STT3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STT3B fusion protein Ag2039
• Vollständiger Name: STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 94 kDa
• GenBank-Zugangsnummer: BC015880
• Gene symbol: STT3B
• Gene ID (NCBI): 201595
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STT3B is a component of the N-oligosaccharyl transferase (OST) enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity

Publikationen

Species	Application	Title
human	WB	Nat Chem Biol; Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.; Authors - Cecilia Lopez-Sambrooks
human	WB	J Cachexia Sarcopenia Muscle; Comparative proteomic analyses of Duchenne muscular dystrophy and Becker muscular dystrophy muscles: changes contributing to preserve muscle function in Becker muscular dystrophy patients.; Authors - Daniele Capitanio
human	WB,CoIP	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright
human	WB	MBio; Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.; Authors - David L Lin
human	WB	EBioMedicine; Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro.; Authors - Hsiang-Chi Huang
human	WB	Cell Chem Biol; Editing N-Glycan Site Occupancy with Small-Molecule Oligosaccharyltransferase Inhibitors.; Authors - Natalia Rinis ; KO Validated