STUB1 Polyklonaler Antikörper

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• KD/KO Validated

STUB1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29751-1-PBS

Synonyme

CHIP, Antigen NY-CO-7, Carboxy terminus of Hsp70-interacting protein, CLL-associated antigen KW-8, E3 ubiquitin-protein ligase CHIP

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  WB analysis using 29751-1-AP (same clone as 29751-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29751-1-AP (STUB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 29751-1-AP (same clone as 29751-1-PBS)

  WB result of STUB1 antibody (29751-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STUB1 transfected HeLa cells. This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29751-1-AP (same clone as 29751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29751-1-AP (same clone as 29751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

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  IHC staining of rat lung using 29751-1-AP (same clone as 29751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 29751-1-AP (same clone as 29751-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29751-1-AP (STUB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29751-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

29751-1-PBS bindet in WB, IHC, Indirect ELISA STUB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STUB1 fusion protein Ag31335
• Vollständiger Name: STIP1 homology and U-box containing protein 1
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC007545
• Gene symbol: STUB1
• Gene ID (NCBI): 10273
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

STUB1, also named as CHIP, SDCCAG7, UBOX1 and NY-CO-7, is a E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. STUB1/CHIP modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. STUB1/CHIP mediates polyubiquitination of CYP3A4.(PMID:10330192) CHIP/STUB1 strongly reduced keratin aggregates through increased degradation of mutant K14. (PMID:20151404) STUB1/CHIP regulates NAD(P)H:quinone oxidoreductase 1 (NQO1) accumulation in aged brain, a process impaired in certain Alzheimer patients.(PMID:21220432 )