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Syntaxin 12 Rekombinanter Antikörper

Syntaxin 12 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242546H2

Kat-Nr. : 85069-4-PBS

Synonyme

242546H2, STX12, Syntaxin-12

Filter:
  • Western Blot (WB) analysis of various lysates using Syntaxin 12 Recombinant antibody (85069-4-RR)

    WB analysis using 85069-4-RR (same clone as 85069-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85069-4-RR (Syntaxin 12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85069-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Syntaxin 12 Recombinant antibody (85069-4-RR)

    IHC staining of human placenta using 85069-4-RR (same clone as 85069-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85069-4-RR (Syntaxin 12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85069-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Syntaxin 12 Recombinant antibody (85069-4-RR)

    IF Staining of A431 using 85069-4-RR (same clone as 85069-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Syntaxin 12 antibody (85069-4-RR, Clone: 242546H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85069-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85069-4-RR

    Affinity and Kinetic Characterization of 85069-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85069-4-RR against Human Syntaxin 12 were performed. The affinity constant is 1.05 nM.

Geprüfte Anwendungen

Produktinformation

85069-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Syntaxin 12 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Syntaxin 12 fusion protein Ag5532
Vollständiger Name syntaxin 12
Berechnetes Molekulargewicht 32 kDa
Beobachtetes Molekulargewicht39 kDa
GenBank-ZugangsnummerBC046999
Gene symbol Syntaxin 12
Gene ID (NCBI) 23673
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during the degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).