Syntaxin 12 Rekombinanter Antikörper

Syntaxin 12 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242546H2

Kat-Nr. : 85069-4-RR

Synonyme

242546H2, STX12, Syntaxin-12

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  WB analysis using 85069-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85069-4-RR (Syntaxin 12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 85069-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85069-4-RR (Syntaxin 12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 85069-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Syntaxin 12 antibody (85069-4-RR, Clone: 242546H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85069-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85069-4-RR against Human Syntaxin 12 were performed. The affinity constant is 1.05 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85069-4-RR bindet in WB, IHC, IF/ICC, ELISA Syntaxin 12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Syntaxin 12 fusion protein Ag5532
• Vollständiger Name: syntaxin 12
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC046999
• Gene symbol: Syntaxin 12
• Gene ID (NCBI): 23673
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during the degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).